Fig. 7.

(A and B) Drawings of frontal sections through MPON and VTA showing lesion and PRV/CTb injection sites. (A) Schematic outlines of ibotenic acid lesion sites in MPON as defined by PRV immunoreactivity from four animals. Numbers above each drawing indicate distance (mm) posterior to bregma. (B) Schematic outlines of PRV/CTb injection sites in VTA as defined by CTb immunoreactivity from the same four animals described in A. Numbers at left indicate distance (mm) posterior to bregma. (C, left) Photomicrographs of frontal sections of PRV labeling in intact and MPON-lesioned animals 48 h following PRV/CTb injection into VTA. (C, right) PRV labeling of nucleus accumbens 48 h post-VTA injection in intact and MPON-lesioned animals. Lesion of the MPON did not significantly change PRV labeling in the nucleus accumbens, another direct VTA afferent. Midline is center in C, left, and the right edge of image in C, right. 3 V, third ventricle; OC, optic chiasm; AC, anterior commissure. Scale bars, 200 μm.