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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Oct 20;32(1):65–73. doi: 10.1161/ATVBAHA.111.239137

Figure 2. Bone marrow transplantation does not change the host atherosclerotic phenotypes.

Figure 2

A. Oil red O staining showing atherosclerotic lesions for aortic arch from PparγΔMϕ/Ldlr−/− mice. Mice were fed a high cholesterol diet and aortic arch was dissected for en face oil red O staining.

B. PCR analysis for Pparγ alleles of peripheral blood from irradiated PparγF/F/Ldlr −/− mice transplanted with PparγΔEC/Ldlr−/− bone marrow or from irradiated PparγΔEC/Ldlr−/− mice transplanted with PparγF/F/Ldlr−/− bone marrow. DNA was extracted from 100 μl of peripheral blood 4 weeks after bone marrow transplantation. The PCR product for the PparγF/F allele is 285 bp and for the PparγΔEC allele is 400 bp

C. Lesion analysis in aorta from PparγF/F/Ldlr−/− mice transplanted with PparγΔEC/Ldlr−/− bone marrow or from PparγΔEC/Ldlr−/− mice transplanted with PparγF/F/Ldlr−/− bone marrow. Mice were treated with high cholesterol diet for 4 weeks after confirming fully chimerism and oil red O staining and quantification of lesion areas were performed.

D. Lesion area in the aortic root from PparγF/F/Ldlr−/− mice transplanted with PparγΔEC/Ldlr−/− bone marrow or from PparγΔEC/Ldlr−/− mice transplanted with PparγF/F/Ldlr−/− bone marrow. **P<0.01.