A. Photographs of ears after treatment with mustard oil (15 min) and vascular perfusion, showing relative amount of extravasated Evans blue tracer.
B. Spectrophotometric measurement of amount of extravasated Evans blue in mouse ears 15 min after topical application of mustard oil. *P<0.05 compared with controls.
C. Expression of genes related with adherens junctions and tight junctions in ECs. NS indicated no statistic difference.