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. 2013 Mar 6;4(2):176–190. doi: 10.3945/an.112.003210

Figure 2.

Figure 2

Effect of zinc on nuclear transcription factor κB (NF-κB) activation in the human monocytic leukemia cell line (THP-1) after oxidized LDL (oxLDL) or phorbol myristate acetate (PMA) stimulation. Zinc-deficient (Zn−) THP-1 cells and zinc-sufficient (Zn+) THP-1 cells were used for the measurement of NF-κB activation by electrophoretic mobility shift assay (EMSA) (A) and luciferase reporter gene assay (B). Effect of zinc on NF-κB activation in human aortic endothelial cells (HAECs) after oxLDL stimulation. Zn− HAECs and Zn+ HAECs were used for the measurement of NF-κB activation by EMSA (C) and luciferase reporter gene assay (D). *P < 0.05 for Zn− compared with Zn+ (Values are SD; n = 3). AFU, arbitrary fluorescent unit/β-galactosidase U/100 μg protein; P.C., positive control; C.C., competition control. Reproduced with permission from (46).