Table 1.
Primers used for the amplification of H. pylori genes.
| Primer | 5′-3′ sequence | Product size (bp) | Annealing (°C) |
|---|---|---|---|
| CagA-F | AATACACCAACGCCTCCAAG | 400 | 55 |
| CagA-R | TTGTTGGCGCTTGCTCTC | ||
|
| |||
| VacA-3.AS | GCCGATATGCAAATGAGCCGC | 678 | 66 |
| VacA-1.SE | CAATCGTGTGGGTTCTGGAGC | ||
|
| |||
| IceA1-F | CGTTGGGTAAGCGTTACAGAATTT | 558 | 56 |
| IceA1-R | TCATTGTATATCCTATCATTACAAG | ||
|
| |||
| IceA2-F | GTTGTCGTTGTTTTAATGAA | 120 | 50 |
| IceA2-R | GTCTTAAACCCCACGATTAAA | ||
|
| |||
| HPU1 | GCCAATGGTAAATTAGTT | 411 | 45 |
| HPU2 | CTCCTTAATTGTTTTTAC | ||
|
| |||
| GlmM-F | AAGCTTTTAGGGGTGTTAGGGGTTT | 294 | 55 |
| GlmM-R | AAGCTTACTTTCTAACACTAACGC | ||