Table 1.

Sources, dilution, distribution, cut-offs point, and pretreatment used for revalidation.

Antibody	Clone	Source	Dilution	Distribution	Scoring System	Cut-offs	Pretreatment	Positive control	Negative control
Bcl-2	124	Dako-Cytomation	1 : 100	Cytoplasm	% of positive cells	>10% (positive)	Antigen retrieval microwave	Normal breast acini	Omitting the antibody
BRCA1	Ab-1 (MS110)	Calbiochem	1 : 150	Nuclear	% of positive cells	<25% (negative)	Antigen retrieval microwave	MCF 7 cells	Omitting the antibody
Ck5/6	M7237	Dako-Cytomation	1 : 60	Cytoplasm	% of positive cells	≥10% (positive)	Antigen retrieval microwave	Known case of CK56 BC	Omitting the antibody
E-cadherin	NCH-38	Dako-Cytomation	1 : 100	Cytoplasm and membrane	% of positive cells	≥100 H score (positive)	Antigen retrieval microwave	Normal gastric mucosa	Omitting the antibody
EGFR	31G7	Novocastra	1 : 30	Membrane	% of positive cells	≥10% (positive)	Not required	Myoepithelial cells of normal duct in normal mammary gland	Omitting the antibody
erbB2	Polyclonal	Dako-Cytomation	1 : 100	Membrane	Table 2.1	Table 2.1	Not required	Known case of erbB2 strong BC expression	Omitting the antibody
ER	1D5	Dako-Cytomation	1 : 200	Nuclear	% of positive cells	≥0 (positive)	Antigen retrieval microwave	Normal breast acini	Omitting the antibody
Ki-67	MIB1	Dako-Cytomation	1 : 25	Nuclear	% of positive cells	<10% (low)	Antigen retrieval microwave	Human tonsil tissue	Omitting the antibody
P-cadherin	NCL-P-cad	Novocastra	1 : 200	Cytoplasm	% of positive cells	≥5% (positive)	Antigen retrieval microwave	Known case of P-cadherin strong BC expression	Omitting the antibody
PgR	PgR	Dako-Cytomation	1 : 150	Nuclear	% of positive cells	≥0 (positive)	Antigen retrieval microwave	Normal breast acini	Omitting the antibody
p21	EA10	Abcam	1 : 25	Nuclear	% of positive cells	≥10% (positive)	Antigen retrieval microwave	Normal breast acini	Omitting the antibody
p53	DO7	Novocastra	1 : 50	Nuclear	% of positive cells	>10% (negative)	Antigen retrieval microwave	Normal breast acini	Omitting the antibody