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Electrostatic energy for 20-mer PNA-DNA or DNA-DNA duplexes using the charged rod-like model under the attractive Gouy-Chapman potential above the positive surface potential (Φ0 = 50 mV). Concentrations of monovalent salt in solution are 15 mM (rinse buffer: 0.1× DPBS) and 750 mM (hybridization buffer: 5× SSC).
