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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Biomaterials. 2013 Apr 1;34(20):4839–4848. doi: 10.1016/j.biomaterials.2013.03.021

Figure 3. Effect of PLL block length and modifying siRNA with 3’-cholesterol on the displacement of siRNA from polyplexes of PLL-PEG(5K) in high serum concentrations.

Figure 3

siRNA (siCtrl: 5’- UGG UUU ACA UGU CGA CUA A - 3’ with 3’-UU overhangs) or Chol-siRNA (siCtrl modified with 3’-cholesterol on the sense strand) was incubated with PLL-PEG(5K) in HEPES buffer at room temperature for 30 min at the minimum N/P ratio required for complexation. Polyplexes were then incubated in the presence or absence of 90%v/v murine serum at 37°C for the indicated time and resolved with or without heparin or water soluble cholesterol (*Cholesterol) as described in Fig.1.