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. 2013 Apr 14;2013:183042. doi: 10.1155/2013/183042

Figure 5.

Figure 5

Effect of SB203580 on the expression of COX-2 and TNF-α mRNA and the production of TNF-α and PGE2. ((a) and (b)) The levels of COX-2 and TNF-α mRNA were determined using semiquantitative RT-PCR or real-time PCR. (a) The levels of TNF-α and PGE2 were determined using ELISA and EIA on culture supernatants from RAW264.7 cells that had been treated with SB203580 (20 μM) and LPS (1 μg/mL) for 6 h (TNF-α) or 24 h (PGE2) h. *P < 0.05 and **P < 0.01 compared to control.