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. 2013 May 5;3(2):124–140.

Figure 1.

Figure 1

Altered architecture of the spleen in MyD88-deficient mice. The macroscopic phenotype of spleens isolated from wild type (wt), Btk- (Btk-ko) or MyD88-single mutant mice (MyD88-ko) as well as double-deficient mice (DKO) was determined by (A) photography and (B) analysis of cell number using a ViCell XR Cell Viability Analyzer after lysis of erythrocytes. Data presented are mean values (±SD) (n=11). C: Spleens of each genotype were embedded in paraffin and processed for HE-staining. Parameters of follicle architecture like (D) follicle number, (E) follicle size and (F) follicle size distribution were analyzed using light microscopy and ImageJ software. Data presented in D and E are mean values (±SD) (n=5). Data presented in F are mean values (±SEM) (n=5). *P ≤ 0.05; **P ≤ 0.005; ***P ≤ 0.005. n represents the number of biological replicates.