Figure 2.

Reduced myeloid and lymphoid cell populations in spleen of MyD88-deficient mice. Spleens of wild type (wt), Btk-deficient (Btk-ko), MyD88-deficient mice (MyD88-ko) and double-deficient mice (DKO) were analyzed by flow cytometry for the frequency of myeloid and lymphoid cell subpopulations. A-G, I-K: Cell suspensions of spleens from mice of each genotype were counted using a ViCell XR Cell Viability Analyzer after lysis of erythrocytes and stained for (A) myeloid cells (CD11b⁺), and (B) B cells (B220⁺) as well as different myeloid subpopulations like (C) granulocytes (CD11b⁺/Ly6G⁺), (D) monocytes (CD11b⁺/Ly6C⁺) (E) macrophages (CD11b⁺/F4/80⁺) and (F) dendritic cells (CD11b⁺/CD11c⁺/MHC II⁺) (n=11 for wt, Btk-ko, DKO; n=7 for MyD88-ko). G: B cells were stained for IgM as well as IgD surface expression (B220⁺/IgM/IgD) and the peripheral B cell subpopulations were analyzed as follows: (I) newly formed B cells (CD21^low/CD23^low), (J) follicular B cells (CD21^int/CD23^high) and (K) MZB cells (CD21^high/CD23^low) (n=10). Data presented are mean values (±SD). Additionally, spleens of each genotype were analyzed by immunofluorescence staining for IgM and IgD (H) as well as for metallophilic macrophages (MOMA) mainly found in the marginal zone of splenic follicles (L). *P ≤ 0.05; **P ≤ 0.005; ***P ≤ 0.0005. n represents the number of biological replicates.