Figure 3.

Decreased myeloid cell populations in the bone marrow of MyD88-deficient mice. Bone marrow cells were isolated from femurs of wild type (wt), Btk-deficient (Btk-ko), MyD88-deficient (MyD88-ko) as well as double-deficient mice (DKO) and analyzed by flow cytometry for the frequency of hematopoietic cell lineages and myeloid subpopulations. A: Cell numbers of whole bone marrow cells per femur were determined using a ViCell XR Cell Viability Analyzer and cells were stained for (B) erythroid lineage (Ter119⁺), (C) lymphoid lineage (B220⁺) as well as (D) myeloid lineage (CD11b⁺). The myeloid subpopulations were further analyzed by staining for (E) monocytes (CD11b⁺/Ly6C⁺) and (F) granulocytes (CD11b⁺/Ly6G⁺). Additionally, the maturation status of granulocytes in the bone marrow can be distinguished by the expression level of CD11b and Ly6G. The different maturation stages were (G) myelocytes (CD11b⁺/Ly6G^low), (H) immature granulocytes (CD11b^med/Ly6G^high) and (I) mature granulocytes (CD11b^high/Ly6G^high). Data presented are mean values (±SD) (n=5). *P ≤ 0.05. n represents the number of biological replicates.