Figure 4.

Apparent reduction of lymphoid cell populations in the bone marrow of Btk/MyD88-deficient mice. Bone marrow cells were isolated from femurs of wild type (wt), Btk-deficient (Btk-ko), MyD88-deficient (MyD88-ko) as well as double-deficient mice (DKO) and analyzed by flow cytometry for the frequency of B cell subpopulations. Cell numbers of whole bone marrow cells per femur were determined using a ViCell XR Cell Viability Analyzer and cells were stained for (A) entire B cell population (B220⁺), (B) pro/pre B cells (B220^med/IgM^-), (C) immature B cells (B220^med/IgM^med), (D) transitional B cells (B220⁺/IgM^high) as well as (E) recirculating B cells (B220^high/IgM⁺). Data presented are mean values (±SD) (n=6). *P ≤ 0.05; **P ≤ 0.005; ***P ≤ 0.0005. n represents the number of biological replicates.