Figure 6.

Reduced proliferation of LT-HSC in the bone marrow of MyD88-deficient mice. Proliferation of hematopoietic stem and progenitor cell population as well as granulocyte subpopulations was determined by use of BrdU-incorporation. Bone marrow cells obtained from femurs of wild type (wt), Btk-ko, MyD88-ko and Btk/MyD88-deficient mice (DKO) after 2 consecutive days of BrdU administration were analyzed by flow cytometry for percentage of BrdU⁺ cells. A: LT-HSC (lin^- Sca1⁺ Kit⁺ CD48^- CD150⁺), (B) multipotent progenitors (lin^- Sca1⁺ Kit⁺ CD48^- CD150^-), (C) common lymphoid progenitors (lin^- Sca1^med Kit^med IL-7Ra⁺), (D) common myeloid progenitors (lin^- Sca1^- Kit⁺ CD34⁺ CD16^med), (E) granulocyte-macrophage progenitor (lin^- Sca1^- Kit⁺ CD34⁺ CD16^high) and (F) megakaryocyte-erythrocyte progenitor (lin^- Sca1^- Kit⁺ CD34^- CD16^-). The analyzed granulocyte maturation stages were (G) myelocytes (CD11b⁺/Ly6G^low), (H) immature granulocytes (CD11b^med/Ly6G^high) and (I) mature granulocytes (CD11b^high/Ly6G^high). Data presented are mean values (±SD) (n=6). **P ≤ 0.005; ***P ≤ 0.0005. n represents the number of biological replicates.