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. 2013 May 5;3(2):181–185.

Figure 1.

Figure 1

A. Selected HRMA results from cell line dilutions testing analytical sensitivity.Melting curves from HRMA sensitivity analysis. Not all dilutions are shown. Dilutions containing theoretical allelic content of V600E BRAF mutation. Yellow: 50% allelic content; Blue, 25% allelic content; Pink: 10% allelic content; Skin, 5% allelic content (note 10% and 5% essentially indistinguishable); Black: 2.5% allelic content; Red: Control 0% allelic content (Parental cell line); B. Representative HRMA results from sample set. Melting curves from HRMA of several random samples with positive and negative sample controls. Red: Positive sample containing theoretical allelic content of V600E BRAF mutation at 25% (Melanoma with >60% neoplastic cell content); Brown: Negative control sample a) BRAF wild type Melanoma sample containing >60% neoplastic cells; Green: Negative control sample b) BM aspirate from a patient with no diagnosed malignancy; Yellow: Representative Study Sample 1; Grey: Sample 5; Blue: Sample 13; Black: Sample 18; Pink: Sample 26.