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. 2013 May 5;3(2):165–173.

Figure 3.

Figure 3

PCR results of investigated IKZF1 deleted patients. Results of the multiplex PCR in different patient subgroups are displayed. Left: multiplex PCR reaction A is able to readily identify IKZF1 Δ2-3 leukemia patients. Middle: multiplex PCR reaction B was used to characterize IKZF1 Δ2-8, IKZF1 Δ4-1 and IKZF1 Δ4-8 leukemia patients. Right: two examples of our unbiased screening of 88 BCP-ALL patients revealed two patients with both IKZF1 alleles partially deleted: both patients (Pts #47 and #65) displayed an IKZF1 Δ4-7 and an IKZF1 Δ4-8 deletion. Lane 1 in panel “Δ4-8” was a negative control (Pt #4). Patient #4 was defined by MLPA analysis to carry an IKZF1 Δ1-8 deletion, and thus, exhibits a larger deletion of chromosome 7. Such patients can neither be positively analyzed by MP-PCR nor by LDI-PCR. Lane 3 in panel “Δ2-8” represents patient #3 that was identified to carry the IKZF1-COBL fusion. Therefore, this patient fails to show any amplification by MP-PCR.