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(A) HPLC chromatogram of the urinary sample after hydrolysis with sulfatase and glucuronidase collected from 6-gingerol treated mice; (B–D) MS2 (positive ion) spectra of peaks at 20.90, 18.15 and 18.79 min in the enzymatic deconjugated mouse urine samples and MS2 (positive ion) spectra of authentic 6-gingerol, (3R,5S)-6-gingerdiol, and (3S,5S)-6-gingerdiol.
