Figure 1. BM cells are recruited at the periphery of pulmonary metastatic lesions.
Wild type mice were lethally irradiated and transplanted with GFP+ BM. Following stable BM engraftment RFP expressing LLC cells were implanted in the flank of these mice. Following primary tumor growth, LLCs metastasized to the lungs. Immunohistochemical evaluation of the lung sections showed presence of BM-derived GFP+ cells, CD31+ mature vessels (magenta) in the RFP+ metastatic lesions. The dotted line separates the host tissue from the metastasis. DAPI was used to stain the nucleus of all cells. (Image courtesy of Dr. Dingcheng Gao, Weill Cornell University Medical Center)
