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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Anticancer Drugs. 2013 Jun;24(5):484–493. doi: 10.1097/CAD.0b013e32835ffdbb

Figure 2. Effect of EHMT2 inhibition on H3K9Me2 level.

Figure 2

(A) LA1-55n cells were treated with BIX-01294 for 24 h at the concentration of 5 μg/ml. The effect of EHMT2 inhibition on the expression of H3K9me2, H3K27Me2, and H3 proteins in LA1-55n cells was determined by Western blot using antibody against H3K9Me2, H3K27Me2, and H3. β-actin was used as an endogenous control. (B) Summary data show BIX-01294 treatment at concentration of 5 μg/ml decreased the H3K9Me level. (C) Summary data show BIX-01294 treatment at concentration of 5 μg/ml does not affect the expression level of H3K27Me2. (D) LA1-55n cells were treated with BIX-01294 at the concentration of 2.5 μg/ml for 24 h. Total RNA was isolated, and real-time PCR was performed to determine the expression of EHMT2. β-actin was used as an endogenous control.