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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Anticancer Drugs. 2013 Jun;24(5):484–493. doi: 10.1097/CAD.0b013e32835ffdbb

Figure 3. Effect of EHMT2 inhibition on cell cycle progression.

Figure 3

(A) Cell cycle distribution was determined by flow cytometric analysis. LA1-55n cells were treated with either vehicle or BIX-01294 (2.5 μg/ml) for 24 h, and subjected to flow cytometric analysis. (B) The expression of cyclin D1, p53 and PCNA was determined by Western blot analysis in LA1-55n cells in the presence or absence of BIX-01294 (2.5 μg/ml). β-actin was used as a loading control. (C) p21 expression was examined by real-time PCR in LA1-55n cells in the presence or absence of BIX-01294 (2.5 μg/ml). β-actin was used as an endogenous control.