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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Anticancer Drugs. 2013 Jun;24(5):484–493. doi: 10.1097/CAD.0b013e32835ffdbb

Figure 6. Effect of EHMT2 inhibition on global DNA methylation level.

Figure 6

Stock solutions of 2′-deoxycytidine and 5-methyl-2′-deoxycytidine were prepared in water. An eight-point stock mixture of a standard was carefully prepared to give an exact known concentration ratio of 2′-deoxycytidine and 5-methyl-2′-deoxycytidine. DNA methylation was determined by LC/MS. Genomic DNA was isolated from control and LA1-55n cells treated with 5 μg/ml BIX-01294 for 24h (A) or 48 h (B) respectively. DNA was then subjected to digestion with nuclease P1, venom phosphodiesterase I, and alkaline phosphatase respectively. The concentration of 2′-deoxycytidine and 5-methyl-2′-deoxycytidine in each sample was calculated from the standard curve. Each DNA sample was analyzed in triplicate. * represents the statistical significant difference (p<0.01) between the vehicle- and BIX-01294-treated cells.