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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Feb 18;190(6):2485–2489. doi: 10.4049/jimmunol.1203208

Figure 2. Cell cycle and LC gene rearrangement analysis of C’-1 and C’-2 stage cells.

Figure 2

(A) qRT-PCR analysis of FACS sorted cells was performed to detect RAG2 (RAG2), PIM2 (PIM2) and ccnd3 (Cyclin D3) transcripts. Data is a composite of 3 independent experiments (n=6). (B) Detection of LC gene (Vκ-Jκ) rearrangements in genomic DNA isolated from FACS sorted cells, as indicated. IL-2 gene was used as a loading control, WT splenic DNA was used as a positive control, and Rag-1-deficient pro-B cell DNA was used as a negative control. Data shown is representative of 4 independent experiments (n=4).