Immunolocalization of phosphorylated HSP27 in wounded central cornea at 0 days (A), 3 days (C), 7 days (E), and 14 days (G) after epithelial debridement and merged images with propidium iodide staining (B, D, F, and H), respectively. Phosphorylated HSP27 was not detected on day 0 because of the absence of epithelium (A and B). On day 3, phosphorylated HSP27 was localized to the superficial and basal epithelial layers, and the expression intensity was similar in all epithelial layers (C and D). After 7 days, phosphorylated HSP27 expression in the basal epithelium was less than that in the superficial epithelium (E and F). After 14 days, phosphorylated HSP27 was localized only to the superficial epithelium as in unwounded corneas (G and H). Scale bar = 20 μm.