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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Hepatology. 2013 Feb 15;57(3):1098–1106. doi: 10.1002/hep.26146

Figure 6. Long-lived Little mice contain high levels of FXR and are resistant to the DEN-mediated cancer.

Figure 6

A. The elevation of FXR in livers of 24-36 month-old mice. Upper image shows Western blotting with proteins from livers of WT and Little mice of different ages. Nuclear extracts from FXR/SHP KO (DKO) livers serve as negative control. Bottom image shows Western blotting with five additional 24-month-old Little mice. B. Levels of FXR were calculated as ratios to β-actin. Bar graphs show a summary of multiple experiments with 9 mice of each genotype. C. Typical pictures of livers of WT and Little mice 35 weeks after DEN injection. Circles show the size of the tumor nodules. D. H&E staining of livers of WT and Little mice after DEN treatments. A typical picture of livers of WT and Little mice treated with DEN is shown. E. BrdU uptake in livers of WT and Little mice treated with DEN. Arrows show BrdU positive hepatocytes. F. Bar graphs show a summary of experiments with two animals of each genotype.