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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Clin Immunol. 2012 Nov 15;146(1):46–55. doi: 10.1016/j.clim.2012.11.003

Figure 4. Multiple rounds of VH replacement generate IgH genes with extremely long CDR3 to encode PGT antibodies.

Figure 4

A. Amino acid sequence analysis of the CDR3 regions of PGT antibodies encoded by VH replacement products. The amino acids contributed by the VH replacement “footprints” are underlined. Positively charged residues are highlighted in red; negatively charged residues are highlighted in blue. B. Frequencies of none charged, positively charged, and negatively charged amino acids encoded by the identified VH replacement products from control IgH gene sequences or IgH genes encoding PGT antibodies. The differences of charged residues in these two groups of VH replacement footprints encoded amino acids were compared and showed no significant difference. C. Structural analysis of IgH CDR3 of PGT antibodies. The complex structure (PDB ID: 3TYG) shows the relative positions of gp120 (gray) and PGT128 antibody (blue and orange). Solved or predicted structures of the IgH CDR3 regions in antibodies PGT121, 122, 123, 127, 128, 130, and 141 are shown. The yellow arrows represent β-strand conformation, which are located at both ends of the CDR3 regions. Positively charged residues are shown in red and negatively charged residues are shown in blue.