Table 1.
Identification of VH replacement products in IgH genes encoding anti-HIV antibodies.
| Sequence ID | VH | VH | P | N1 | P | DH | DH | |
|---|---|---|---|---|---|---|---|---|
| Anti-gp41 | EU794402 | IGHV1-69 | tgtacgaga | gatagaagtagggtt | cgatattttgaccgg | IGHD3-9 | ||
| EU794399 | IGHV1-69 | tgtgcgaga | gtcgtctcaagtgcccggggacgtcaaatatcacgc | atgttttggga | IGHD3-10 | |||
|
| ||||||||
| Anti-V3 loop | EU794433 | IGHV3-7 | tgtgcgag | gtggatcggggaaccccaaaaagaagga | tactactatgat | IGHD3-22 | ||
| EU794439 | IGHV4-59 | tgtgcg | cggattagtgttgttgtcctg | ccagctgttttccc | IGHD2-2 | |||
| EU794437 | IGHV5-51 | tgtgcgaga | ctaggatatg | atgataccagtgattctt | IGHD3-22 | |||
| EU794436 | IGHV1-18 | tgtgtgagag | taagagata | a | tgaacagggagactac | IGHD4-17 | ||
| EU794422 | IGHV4-59 | tgtgcgaga | acccagaccg | gcagcggctggtac | IGHD6-13 | |||
| S77136 | IGHV3-11 | tgtgcgagag | gacgaggaggc | tattatgattacatttgggggagt | IGHD3-16 | |||
| EU794425 | IGHV2-5 | tgtgttcgg | acatatttca | atggaaatagtggttat | IGHD3-22 | |||
| EU794427 | IGHV5-51 | tgtgcgag | gacatat | tactatgaagatagt | IGHD3-22 | |||
| EU794414 | IGHV5-51 | tgtacgaga | tggtttagcgac | gagacgtctacaatt | IGHD5-24 | |||
|
| ||||||||
| Anti-gp120 | Z35118 | IGHV2-26 | tgtg | atcagatgggcgggtc | gatgttgttgtgg | IGHD2-15 | ||
| Z35126 | IGHV4-31 | tgtgcgaaag | cagacggc | tattacgatttttggagtg | IGHD3-3 | |||
| U82950 | IGHV1-69 | tgtgc | taaagaaggagagcaggtgg | gatattttgactgg | IGHD3-3 | |||
| AF086903 | IGHV3-30 | tgtgcgaga | ggcgcgatgaaagac | tacgatttctggagtggtta | IGHD3-3 | |||
| U82767 | IGHV1-69 | tgtgcga | cagatggctccaggctgtctacctcggcctt | cgatttttggagtggtaat | IGHD3-3 | |||
| S67982 | IGHV1-69 | tgtgcg | gccgtcagg | attgtaccagtccc | IGHD2-2 | |||
| S67984 | IGHV2-5 | tgtgcataca | cccttctctctgtgataag | tacgacttgggaag | IGHD3-3 | |||
| U82942 | IGHV1-69 | tgtgcgcga | cactctggaagatacat | taacgggaacta | IGHD1-7 | |||
|
| ||||||||
| CD4i | 17b-H | IGHV1-69 | tgtgcggga | gtgtacgagggagaggcggacgagggggaa | tatgataataatgg | IGHD3-22 | ||
| c12-H | IGHV1-69 | tgtgcgagaga | t | gtaggccccgactgggataacgatg | attactatgatcgtagtgg | IGHD3-22 | ||
| AY515006(23e) | IGHV1-69 | tgtgcgaga | ggggggcctgtgttgcagagtg | acgatttttggaatggttat | IGHD3-3 | |||
| M16-H | IGHV1-69 | tgtgcga | ccgcgtctgaagccactga | gaatgattattat | IGHD3-3 | |||
| SB1-H | IGHV1-69 | tgtgcga | cccggaatccgaacga | gtattacgatg | IGHD3-10 | |||
| AY539808(X5) | IGHV1-69 | tgtgcgagaga | t | tttggccccgactgggaagacggtg | attcctatgatggtagtgg | IGHD3-22 | ||
| AY515008(47e) | IGHV1-69 | tgtgcgaaag | gaggggagg | acggtgactac | IGHD4-17 | |||
| AY515012(E51) | IGHV1-69 | tgtgcgag | taattctattgccggagtagcagctgccgga | gactacgctgactac | IGHD4-17 | |||
|
| ||||||||
| PGT | PGT121H | IGHV4-59 | tgcgcgaga | acactgcacgggaggagaatttatggaatcgttgccttcaat | gagtggtt | IGHD3-10 | ||
| PGT122H | IGHV4-61 | tgcgcga | caacaaaacacgggaggaggatttatggcgtcgttgccttcaaagagtggtt | cacctat | IGHD3-16 | |||
| PGT123H | IGHV4-59 | tgtgcg | cg | agcacttcacgggaagaggatttatgggatagttgccctcgga | gagttgtt | IGHD3-16 | ||
| PGT125H | IGHV4-b | tgtgcgcga | ttcgacggcgaag | tcttggtct | IGHD4-17 | |||
| PGT126H | IGHV4-b | tgtgcgcga | ttcgacggcgaag | ttttggtgt | IGHD3-22 | |||
| PGT127H | IGHV4-61 | tgtgcgcga | ttcggcggcgaag | ttctagtgt | IGHD3-10 | |||
| PGT128H | IGHV4-b | tgtgcgcga | ttcggcggcgaag | ttttacgct | IGHD4-17 | |||
| PGT130H | IGHV4-39 | tg | cgtacgatccggcggcgacatctta | tattattatg | IGHD1-14 | |||
| PGT131H | IGHV4-39 | tg | cgttcgatccggcggcgacatttta | tactatattg | IGHD2-8 | |||
| PGT135H | IGHV4-34 | tgtgcgaga | caccgacatcatgatatcatgatgttttcatgttggtccctattgcg | ggctggt | IGHD3-16 | |||
| PGT136H | IGHV4-39 | tgtgcgagaca | t | aaatatcat | gatatttt | IGHD2-15 | ||
| PGT137H | IGHV4-39 | tgtgtga | agcacaaatatcatgacattgtca | tggtggt | IGHD1-14 | |||
| PGT141H | IGHV1-8 | tgtacgagagg | ctcaaaacatcgtttgcgagactacgttctctacgatgactacggcttaattaattatcaagagtggaa | tgactaccttga | IGHD4-17 | |||
| PGT142H | IGHV1-8 | tgtacgagagg | ctcaaaacatcgcttgcgagactacgttctctacgatgactacggcttaattaattatcaagagtggaa | tgactaccttga | IGHD4-17 | |||
| PGT143H | IGHV1-8 | tgtacgagagg | ttcaaaacatcgcttgcgagactacgttctctacgatgactacggcttaattaattatcaagagtggaa | tgactaccttga | IGHD4-17 | |||
| PGT144H | IGHV1-8 | tgtaccggagg | ctcaaaacatcgcttgcgagactacgttctctacgatgattacggcctaataaatcagcaagagtggaa | tgactaccttga | IGHD4-17 | |||
| PGT145H | IGHV1-8 | tgtttgaccgg | ctcaaaacatcgcctgcgagattattttctgtacaatgaatatggccccaagagtgg | ggtgactac | IGHD4-17 | |||
a
Potential VH replacement footprints are highlighted in red (no mismatch) or purple (with one mismatch).