Figure 5.
Plasmid abundance in spinal cord correlates with expression. Samples were obtained one day after intrathecal injection of 100 µg of pCpG− [SEAP] (n = 7; ○) or from a naive animal (n = 1; x). The samples consisted of lumbosacral CSF and a 5-mm length of spinal cord (including meninges and attached dorsal root ganglia) surrounding the site where DNA is delivered. CSF was assayed for SEAP concentration and the spinal cord tissue was assayed for the presence of pCpG− [SEAP] via quantitative PCR. CSF SEAP concentration versus plasmid abundance is shown
