Figure 2.

Gel images of labeling studies using purified Akt-PH (fluorescent gel image shown in grey scale). Studies indicated successful labeling of Akt-PH protein by both fluorescein-probe 1 (lane 2) and alkyne-probe 2 (lane 4, after click chemistry post-derivatization) during studies. Additionally, control studies involving no photo-cross-linking (lane 1), or heat denaturation of the protein prior to probe incubation (lanes 3 and 5) yielded no fluorescence, indicating the absence of non-specific labeling. Finally, Coomassie Blue stains indicate that the protein is still present despite the abrogation of probe labeling. Please also see color fluorescence gel scans in Figure S1 of the supplementary information.