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Competition studies involving Akt-PH labeling (fluorescent gel image shown in grey scale). In lanes 1–4, pre-incubation of Akt-PH with excess inhibitor 4b suppressed labeling by probe 2 as visualized by a reduction in fluorescence intensity of the AKt-PH band. In lane 5, the bifunctional lysine lacking the PIP headgroup did not label the Akt protein.
