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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Biochemistry. 2011 Nov 30;50(51):11143–11161. doi: 10.1021/bi201636s

Figure 7.

Figure 7

Labeling of MDA-MB-435 soluble proteome using various conditions (fluorescent gel image shown in grey scale). Studies using fluorescein-probe 1 resulted in significantly stronger labeling in the heat denatured control (lane 2) compared to the normal study (lane 1), indicating that the presence of the fluorophore during labeling is problematic. Studies using probe 2 and post-labeling led to diminished labeling in heat-the denatured control (lane 5, when compared to lane 4). No probe (lane 6) and no light (lane 8) controls negated labeling, and click chemistry ligands TBTA (lane 7) and THPTA (lane 9) yielded similar results. Please also see color fluorescence gel scans in Figure S3 of the supplementary information.