Figure 2.

— Double immunostaining with for CD31 and podoplanin for vessel density analysis. (a) CD31-positive vessels (green). (b) Podoplanin-positive vessels (red). (c) Merge of CD31 and podoplanin staining. (d) Vessel density analysis for a representative CD31-positive sample (n = 30, black circles). The area of the submesothelial compact zone is 0.199 mm², yielding a vessel density of 150.7/mm²). (e) Vessel density analysis for a representative podoplanin-positive lymphatic vessel (n = 3, black arrows). The area of the submesothelial compact zone is 0.209 mm², yielding a vessel density of 14.4/mm²).