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. 2013 Apr 26;6:122. doi: 10.1186/1756-3305-6-122

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Copyright © 2013 Cunha et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vitro differential infectivity of L. infantum strains. BMMo were infected with promastigotes of each strain after 4 days of culture in NNN in a ratio of 10:1 (parasites:macrophage). The kinetics of infection was followed counting (A) the infected cells and (B) the number of parasites per infected cell on fluorescent microscope. To account to the overall parasite load, an infection index (C) was calculated multiplying the individual data from (A) by (B). Statistically significant differences between ST and the other strains were determined with One-way ANOVA followed by Tukey’s multiple comparison test.