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. 2013 May 1;9:90. doi: 10.1186/1746-6148-9-90

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Copyright © 2013 Murray et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Evaluation of candidate LAMP assays. A: Agarose gel (2%) showing the characteristic laddering pattern generated by LAMP for the detection of GTPV isolate Vietnam Ninh Tuan 05 by three different LAMP primer sets: EP32A (P32 gene); Epol (RNA polymerase RPO30 subunit) and ETop (DNA topoisomerase I). Results for negative controls (−) are shown. MW: molecular weight ladder (100 bp). B: Corresponding increase in fluorescence generated for EP32A (○: circle); Epol (△: triangle) and ETop (◊: diamond) LAMP assays using a real-time PCR machine. Representative signal for a negative control (□) well is shown.