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. 2013 May 9;9(5):e1003346. doi: 10.1371/journal.ppat.1003346

Figure 1. The T. annulata schizont surface protein p104 contains an SxIP motif.

Figure 1

(A) Alignment of p104554–593 with the corresponding sequences from human APC, CLASP1, CLASP2, MCAK, MCAF2 and STIM1. The SKIP motif is highlighted in yellow, serines, prolines and basic residues (K/R) in blue. The recently expanded nine-amino acid SxIP motif (SxIP 9AA) defined by Jiang et al. [28] is included for reference. (B) Image showing how mAb 1C12 (green) labels the schizont surface; MTs are stained with anti-tubulin (red). DNA is stained with DAPI (blue). Scale bar = 5 µm. (C) Immunoblot analysis of lysates prepared from COS-7 cells expressing V5-tagged p104 fragments (full-length; p10420–871-V5, C-terminal; p104470–871-V5, or N-terminal; p10420–469-V5). Blots were probed with 1C12 and reprobed with anti-V5.