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. 2013 May 9;9(5):e1003346. doi: 10.1371/journal.ppat.1003346

Figure 2. p104-V5 co-localization with endogenous EB1 at MT plus ends requires an intact SxIP motif.

Figure 2

(A) Image of a COS-7 cell transiently expressing p104-CT-V5, fixed with ice-cold methanol and stained with anti-EB1 (red) and anti-V5 (green); the white rectangle indicates the magnified cytoplasmic region. DNA is stained with DAPI (blue). Scale bar = 10 µm. (B) The N-terminal region of p104 (p104-NT-V5) does not localize to MT plus ends. (C) Mutation of the SKIP motif to SKNN abolishes plus end localization. (D) Image of over-expressed p104-CT-V5 co-localizing with EB1-GFP at bundled MTs in COS-7 cells.