Table 2. Pyrosequencing results, with median methylation of normal tissue shown at bottom.

			SLIT2 pyrosequencing of MSP site					Long range SLIT2
Sample	Tissue type	Location	CpG1	CpG2	CpG3	CpG4	CpG5	Average methylation
Serrated adenomas	SSA/P	Caecum	22%	70% *	35% *	51% *	26% *	10%
	SSA/P	Rectum	35% *	61% *	48% *	48% *	0%	0%
	TSA	Caecum	33% *	33%	29%	0%	21% *	9%
	SSA/P	Rectum	36% *	64% *	49% *	84% *	0%	0%
100% methylated	Human control DNA	-	83% *	88% *	82% *	94% *	91% *	85%
Caecal polyp	Adenoma with low grade dysplasia	Caecum	19%	22%	15%	23%	14%	-
Normal mucosa	Normal mucosa	Ascending colon	29% *	36% *	17%	38%	18%	-
	Normal mucosa	Transverse colon	9%	15%	11%	14%	10%	-
	Normal mucosa	Left colon	16%	29%	0%	32%	17%	-
	Normal mucosa	Descending colon	16%	17%	20%	26%	9%	-
	Normal mucosa	Sigmoid	11%	14%	10%	13%	9%	-
	Normal mucosa	Rectum	14%	14%	13%	17%	8%	-
	Normal mucosa	Distal rectum	11%	19%	18%	36%	13%	-
Cell lines	C80	-	100% *	91% *	93% *	100% *	67% *	-
	HDC57	-	93% *	69% *	67% *	97% *	66% *	-
	LOVO	-	79% *	90% *	90% *	100% *	71% *	-
	C99	-	95% *	90% *	88% *	100% *	74% *	-
	LS123	-	100% *	87% *	94% *	100% *	56% *	-
	CACO2	-	96% *	91% *	94% *	100% *	68% *	-
	HDC82	-	84% *	80% *	92% *	100% *	67% *	-
	COLO741	-	46% *	52% *	67% *	73% *	46% *	-
	LS411	-	40% *	38% *	36% *	42%	23% *	-
	CCK81	-	87% *	90% *	92% *	100% *	70% *	-
	SNU175	-	64% *	60% *	80% *	96% *	64% *	-
Median	Normal tissue		16%	19%	15%	25%	13%	-
SD	Normal tissue		6%	8%	6%	9%	4%	-
2SD+median	Normal tissue		28%	34%	30%	43%	20%

Asterisks indicate hypermethylation (>2SD from median).