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. 2013 May 9;9(5):e1003497. doi: 10.1371/journal.pgen.1003497

Figure 5. Markers of the transition zone persist in spd-3(me85) mutants.

Figure 5

(A) Projections of transition zone and pachytene nuclei from wild type and spd-3(me85) mutants stained with anti-PLK-2 and anti-SYP-1 antibodies, and counterstained with DAPI. Note that both PLK-2 signals and chromosome clustering (seen by DAPI staining) persist until mid pachytene in spd-3(me85) mutants. The WT mid-pachytene nuclei correspond to the region of the germ line in which PLK-2 is no longer seen forming aggregates on the NE, and in which PLK-2 had not started to accumulate on the SC, which occurred in late pachytene nuclei. (B) Projections of transition zone and pachytene nuclei from the indicated genotypes stained with antibodies specific for SUN-1 phosphorylation at Serine 12 and counterstained with DAPI. Note that both SUN-1 pSer12 staining and chromosome clustering persist in mid-pachytene nuclei of spd-3(me85) mutants, and that this persistence is HTP-1 dependent. Scale bar = 5 µm in all panels.