Skip to main content
. 2013 May 9;9(5):e1003497. doi: 10.1371/journal.pgen.1003497

Figure 7. Depletion of cytoskeletal motors impairs SUN-1::GFP mobility.

Figure 7

(A) Projections of transition zone nuclei from worms expressing SUN-1::GFP and subjected to RNAi of the indicated genes encoding components of the dynein motor complex, and showing the formation of numerous small SUN-1 aggregates that contrast to the larger -and fewer- aggregates seen in wild-type nuclei. (B) Plotting of SUN-1 movement following dynein knockdown. dhc-1 RNAi results in a reduction of the distance covered and speeds of SUN-1 aggregates. See legend of Figure 6 for a description of the graphs. (C) Top panel: whole germ line following dhc-1 RNAi stained with DAPI. Bottom panel: projection of the nuclei included in the three regions outlined on the whole-germ line panel. Nuclei are stained with anti-SYP-1 and anti-HIM-8 antibodies and counterstained with DAPI. Some nuclei from zones 1 and 2 show separated HIM-8 signals associated with different tracks of SYP-1. (D) Projection of transition zone nuclei from wild type and spd-3(me85) mutant worms expressing DHC-1::GFP or DNC-2::GFP and stained with anti-GFP antibodies and antibodies specific for serine 8 phosphorylation on SUN-1, and counterstained with DAPI. DHC-1 and DNC-2 localize to SUN-1 aggregates marked by SUN-1 S8Pi in the NE of both wild type and spd-3(me85) mutant nuclei. Scale bar = 5 µm in all panels.