Table 2. HIV in blood cells.

Sample	Date	Lab	Measure	Input	Assay	Detection Limit	False Positive Rate1	Result
PBMC	5/31/11	NIH	HIV DNA	30×106 cells	qPCR for LTR [19]	0.5–2.6 copy/µg	0/72 (<1.4%)	ND2
PBMC	7/5/11	SFVA/UCSF	HIV DNA	106 cells	qPCR for LTR [24], [56]	1–2 copy/reaction [56]	1/130 (0.8%)	1 of 10 wells equivocal
PBMC	11/17/11	Johns Hopkins	HIV DNA	106 cells	qPCR for Gag [25]	1 copy/reaction		ND (≤1 in 106)
PBMC	8/1/11	UCSD	HIV DNA	106 cells	Digital droplet PCR for Pol [26]	0.52 copy/106 cells	7/29 (24%)	ND (≤1 in 106)
PBMC (sorted)	2/29/12	Vaccine Research Center	HIV DNA	6.7×104 CCR5+ cells	Fluorescence- assisted clonal amplification of Env	1 copy/reaction		ND
				7.6×106 CCR5− non-T cells				ND
				3.5×106 CCR5− CD4+ T cells				ND
				2.1×106 other CCR5− T cells				ND
PBMC	5/31/11	NIH	HIV RNA	10×106 cells	Roche Ampliprep [19]	1–2 copy/ml	1/179 (0.6%)	ND (≤1 in 107)
PBMC	7/5/11	SFVA/UCSF	HIV RNA	106 cells	qRT-PCR for LTR [24], [56]	1–2 copy/reaction [56]	0/83 (<1.2%)	ND (≤1 in 106)
PBMC	6/20/11	BSRI	HIV RNA	3×106 cells	TMA [22]	1 copy/ml [22]	6/3515 (0.2%)	ND (≤1 in 3×106)
PBMC (sorted)	2/29/12	Vaccine Research Center	HIV RNA	6.8×104 CCR5+ cells	RNA sequencing	1 read/sample		ND
				8.6×106 CCR5− non-T cells				ND
				3.4×106 CCR5− CD4+ T cells				ND
				4.2×106 other CCR5− T cells				ND
Peripheral CD4+T cells	5/31/11	NIH	Replication compent HIV (IUPM)	1.4×109 cells	Co-culture [27]			ND (≤1 IU/109 cells)
Resting peripheral CD4+ T cells	11/17/11	Johns Hopkins	Replication compent HIV (IUPM)	33×106 cells	Co-culture [28]			ND (≤1 IU/107 cells)

¹Historical, as determined from the number of positive wells out of all wells containing samples from HIV- subjects/donors that had been processed and run along with positive samples.

²ND = not detected.