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. 2013 May 9;9(5):e1003348. doi: 10.1371/journal.ppat.1003348

Figure 3. Knockdown of lef-4 and lef-9 reduces expression of MdBV structural genes.

Figure 3

M. demolitor larvae were injected with ds-eGFP, ds-lef-4 or ds-lef-9 as shown in Figure 2. The ovaries from individual, newly emerged adults wasps were then dissected and total RNA isolated followed by measurement of copy number per ng of total RNA of: (A) vp39, (B) p74, (C) ef1α, (D) dnapolδ. (E) Copy number of DNase-protected MdBV episomal genomic segment B in the ovaries of newly emerged M. demolitor adult females. The ovaries from individual, newly emerged adults wasps were dissected and treated with DNAse. DNA was then isolated and total copy number of episomal genomic segment B determined. Error bars, N values, and statistical significance are indicated as defined in Figure 2.