Figure 1. TaWRKY71-1 are specifically transcribed in wheat leaves with a higher expression level in SR3.

A: The hybridization signals of the probe referring to TaWRKY71-1 in the microarray using wheat seedlings harvested at three-leaf stage. Data present as mean ± standard deviation. B: The sqRT-PCR using two-week old wheat seedlings. A wheat actin gene was used as the internal reference. JN177, a common wheat cultivar; SR3, a wheat introgression cultivar selected from somatic hybrids between JN177 and its close relative grass Thinopyrum ponticum via asymmetric somatic hybridization. 28C, 28 cycles; 38C, 38 cycles.