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. 2013 May 9;8(5):e62455. doi: 10.1371/journal.pone.0062455

Figure 2. Quantification of deer CWD prion by the cervid prion cell assay.

Figure 2

A. Representative wells of an ELISPOT plate showing spots given by triplicate deer 5E9-S1cells exposed to 3-fold serial dilutions of CWD+ inoculate brain homogenate, deer CWD (upper) and Tg(CerPrP-M132)1536+/−-passaged CWD prion isolate 012-09442 (lower), the concentration of brain homogenates (left to right) are: 10−2, 10−2.5, 10−2.9, 10−3.4, 10−3.9, 10−4.4 and 10−4.9. The wells at right are uninfected Deer5E9-S1 cells. B. Responsiveness of Deer5E9-S1 cells to the CWD+ inoculum and Tg(CerPrP-M132)1536+/− mouse -passaged CWD prion isolate 012-09442 were between 10−2 and 10−5. The cells were infected with serial 1∶3 dilutions of homogenates of CWD+ inoculum and subjected to the CPCA. In each case, the mean is derived from experiments performed in triplicate, with error bars indicating the standard errors of the means (SEM).