Figure 3. Structure of RV6-26 Fab bound to RV-DLP.

(A) A representative cryo-micrograph of RV6-26-DLP complexes vitrified in ice over a hole in Quantifoil holey carbon grids. The white boxes indicate complexed particles that were extracted and processed. The inset shows a representative cryo-micrograph of unbound RV-DLP. (B) Surface representation of the resulting 3D structure of RV6-26-DLP complex reconstructed to a 10.9 Å resolution. RV6-26 Fab (yellow) at five-fold axis is indicated by red pentagon (also shown in [C]) Red arrows indicate the location of additional five-fold axes on the structure. The Fabs bound in the pseudo-six-fold axes directly adjacent to the transcriptional pores (blue hexagon [D]) exhibit a different average density representation from those bound at the pseudo-six-fold axes not directly adjacent to the transcriptional pore (brown hexagon [E]).