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. 2013 May 9;8(5):e61023. doi: 10.1371/journal.pone.0061023

Figure 3. Fold change of MUC5AC mRNA measured using real time PCR.

Figure 3

(A) In PBEC(N) on day 28 of ALI culture we observed a repeated measures ANOVA overall p value of p = 0.05. A significant increase in MUC5AC mRNA by stimulation with IL-9 (p = 0.02), IL-9/IL13 combination (p = 0.02) and IL-13 (p = 0.04) was observed. (B) In PBEC(A) a repeated measures ANOVA overall p value of p = 0.00098 was obtained. A significant increase compared with unstimulated was observed only with IL-13 stimulation (p = 0.016). Additionally, there were significant differences in MUC5AC mRNA between IL-9 and IL-13 stimulations (p = 0.01) and between IL-9/IL-13 combination and IL-13 stimulations (p = 0.01).