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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Exp Neurol. 2011 May 18;230(2):302–310. doi: 10.1016/j.expneurol.2011.05.009

Figure 4. Glutamate receptor expression and adaptation to hypoxia.

Figure 4

A) Western blotting for principal NMDA receptor subunits NR1, NR2A, and NR2B from membrane preparations from neurons (DIV 8–10) cultured in 20% O2, 5% O2, and 1% O2 environments. B) Quantitation of relative band strength, normalized to protein loading, from neurons from 20%, 5%, and 1% conditions (n = 3 independent samples; mean ± SD; differences not significant by ANOVA). C) Representative whole cell currents elicited by 1 sec applications of 300 μM NMDA and 10 μM glycine (bar) in cortical neurons (DIV 8–10) cultured in 20%, 5%, or 1% O2 atmospheres. D) Whole cell peak currents (top), fractional block by 1 mM Mg2+ (middle) and fractional desensitization over 1 sec (bottom), in cortical neurons cultured in 20% O2 (n = 9), 5% O2 (n = 8), and 1% O2 (n = 9). Each circle represents a measurement from a single cell, with mean values represented by horizontal bars. Distributions were highly overlapping, and no significant differences were found (p = 0.16, p = 0.94, and p = 0.14, respectively, by ANOVA).