All neurons were cultured under ambient conditions (20% O2), and treated with DMOG (25 μM) from day 0 in culture. Simulated ischemia was applied for 60 min on day 8–10, without (‘IR’) or with added glutamate (‘IR+Glu’), and relative survival compared at 2, 24, and 72 hours. DMOG pre-treatment completely blocked neuronal death during reperfusion. DMOG alone produced no change in relative survival (n = 5, mean ± S.D.; * treatment groups different, p < 0.05 by two-way ANOVA; effects of reperfusion time were not significant).