Table 3. Sequences of oligonucleotide primers used in RT-qPCR.
| Genesymbol | Accessionnumber | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon(bp) | Efficiency | Error1 | Assay ID |
| AI314976 | NM_207219 | GGCGTCACTGACCTCTCCAT | TTGACTCAAACACCTCTTCGAGAA | 97 | 77 | 13.4 | 85973 |
| Aldoa | NM_001177307 | GTGTTGTGGGCATTAAGGTAGATAAG | CATCCAGCCCCTGGGTAGTT | 81 | 95 | 2.9 | 85983 |
| Atp6v0d1 | NM_013477 | ATTTCAACGTGGACAATGGCTACTT | TCTCGCACTGCACCAGGTT | 99 | 88 | 2 | 85993 |
| B1 element | multiple | TGGCGCACGCCTTTAATC | GCTGGCCTCGAACTCAGAAAT | 46–73 | 56 | 1.4 | Unpublished4 |
| B2 element | multiple | CAATTCCCAGCAACCACATG | ACACCAGAAGAGGGCATCAGA | 66–68 | 82 | 1.4 | Unpublished4 |
| B3gnt3 | NM_028189 | CCCCTACGAGATCCTCCTCC | CACGGATAGATTAGCTCGGCA | 181 | 85 | 2.7 | 31982625a12 |
| Fbln1 | NM_010180 | ACCAGGCCGACATCATCTTC | TTCAGGACAGCGTAAAACGGG | 142 | 68 | 1.8 | 6753822a22 |
| Gag@ | multiple | TGGAGTAGGCTGGCCACCTA | CAGCCAAGCCAAACGCTATT | 131 | 79 | 5.3 | 86093 |
| Gm14743 | NM_001126321 | GACGAGTGGTCCCCTGGAG | TGCTTATACCCAGTGACTGTGGTC | 129 | 87 | 4 | 86113 |
| Gsr | NM_010344 | TGACAACATCCCTACTGTGGTCTT | GGGTAAAGGCAGTCGAGTAGATTTTC | 122 | 88 | 0.4 | 86043 |
| Hadhb | NM_145558 | GCTAGAGCTGCACTTTCGGG | GCCACATTGCTTGTTTTCACTTC | 110 | 85 | 2.2 | 21704100a22 |
| Hjurp | NM_198652 | CGGTGCGGATACGAGCTT | CTCTGCGTTCTTAAAATACTCTTCATCT | 187 | 84 | 0.8 | 86003 |
| Hprt | NM_013556 | AGCTACTGTAATGATCAGTCAACG | AGAGGTCCTTTTCACCAGCA | 198 | 82 | 0.5 | 35833 |
| Oaz1 | NM_008753 | GTGGTGGCCTCTACATCGAG | AGCAGATGAAAACGTGGTCAG | 120 | 82 | 1.3 | 7110677a32 |
| Pcdha@ | multiple | TGGAGGAGGCTGGCATTCTA | GGGTTGCCTGGTCCGTATT | 163 | 84 | 8.5 | 86083 |
| Plekha7 | NM_172743 | GAACGCTTCCGTGCTCAAAG | GATCGACAAACAAGGCATCCT | 103 | 86 | 0.1 | 27370088a22 |
| Rpl4 | NM_024212 | CCGTCCCCTCATATCGGTGTA | GCATAGGGCTGTCTGTTGTTTTT | 147 | 84 | 0.4 | 30794450a12 |
| Rps29 | NM_009093 | CAACCGCCACGGTCTGAT | CATTCAAGGTCGCTTAGTCCAACT | 110 | 86 | 1.2 | 86023 |
| Slc52a3 | multiple6 | ACCAGGGACCACCTTGAACACTA | CAGCAGCACGTCAGGAAAGAC | 159 | 76 | 2.3 | 86103 |
| St6galnac1 | NM_011371 | GCCATCCACCATGACGAGAT | GCACTTGCGTCATTCTTCCA | 159 | 91 | 4 | 6755510a12 |
| Tmem14c | NM_025387 | CTGTCTCAGGATCCCAGGAATGT | CAAACTGGCTCCTGCGATTAA | 129 | 83 | 4.7 | 86033 |
| Tspan15 | NM_197996 | CGGCAGAAATACAAAACCCTGG | CAGAAGGCACAGGTTGTCC | 132 | 95 | 0.6 | 13278031a22 |
| Zfp598 | NM_183149 | GGTGCTCTACCAAGATGCGG | GGATGGGGATCAGGGCAAA | 117 | 87 | 0.6 | 34147169a12 |
| Zfyve19 | multiple5 | GCTGTCAAGTTCACCCTCTTCAA | GGCTCGGCCACAATTCTTACA | 57 | 90 | 5.4 | 86053 |
| Ube2v1 | NM_023230 | TGATAATTGGACCTCCACGAACAA | TCTCGGATCCACCACTCCAT | 158 | 95 | 1.6 | 86063 |
| Usmg2 | AJ290944 | TTAGGGATGCAGAACACGTGAAA | CAACATCAACTCAAGCTAACATAGGAA | 59 | 99 | 2.3 | 86073 |
@: gene cluster.
Pcdha@: Pcdha cluster of genes binding to Pcdha1, Pcdha10, Pcdha11, Pcdha12, Pcdha2, Pcdha3, Pcdha4, Pcdha5, Pcdha6, Pcdha7, Pcdha8, Pcdha9, Pcdhac1 and Pcdhac2.
1
mean squared error of the single data points fit to the regression line given in %.
assay details are accessible in the original publication or in the repositories for primer sequences (2PrimerBank or 3RTPrimerDB).
4
primer sequences provided by Vandesompele, J.
5
accession numbers: NM_001164827 and NM_028054.
6
accession numbers: NM_001164819 and NM_027172.