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. 2013 May 9;8(5):e62828. doi: 10.1371/journal.pone.0062828

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© 2013 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Detection of 5 hmC in HCC cells (Huh7 and PLC/PRF/5) by immunofluorescent staining. The representative photomicrographs were presented. (B) Detection of 5 hmC in the paraffin-embedded formalin–fixed HCC tissues by immunohistochemistry. Representative photomicrographs were presented. (C and D) The level of 5 hmC was examined in a tissuearray containing 146 paraffin-embedded formalin–fixed HCC tissues and paired non-HCC counterparts by immunohistochemistry. The representative photomicrographs of 5 hmC level with high, equal and low in HCC tumors, as compared with non-tumor tissues, were shown (C). The altered level of 5 hmC between HCC and non-tumor tissues in 146 HCC patients were summarized (D) (n = number of cases).