Figure 4. Level of 5 hmC is dynamic changed in an animal model of DEN-induced liver cancer.

DEN was given to rats for 24 weeks for induction of liver cancer. Before induction and different time points after induction, animals were sacrificed and liver tissue samples were collected. (A) Level of 5 hmC was analyzed in the paraffin-embedded formalin–fixed rat liver and tumor tissues by immunohistochemistry. Representative photomicrographs for 5 hmC in liver and tumor tissues were presented. (B) Quantification of 5 hmC level was carried out in liver tissues from normal animals, animals during induction and tumor tissues. (n = 5 animals per group).