Table 1. Genes included in the RT-qPCR assay, their abbreviations and putative function.
Protein name | Abbreviation | Putative functional role |
Complement-component 3 | C3 | Essential for activating the complement system in the immune response [87], [120] |
Nuclear factor Kappa-B, Subunit 2 | Nf-kb2 | Activated in response to environmental stress, pathogens and chemicals [87], [120] |
Hemolytic lectin | Cel3 | Cell surface recognition and metamorphosis [88] |
C type lectin | Ctl | Cell surface recognition, immunity and symbiosis [88], [89] |
Urokinase Plasminogen Activation Receptor | uPar | Serine protease involved in the plasminogen activation system [59], [95] |
Superoxide dismutase | MnSod | Involved in the antioxidant defense system [105] |
Catalase | Cat | Involved in the antioxidant defense system [105] |
Soma ferritin | Sof | Involved in ferroxidase activity and cellular homeostasis by minimising Fe2+ availability for ROS production [100] |
H+ transporting ATPase | H-ATPase | Involved in the ATP synthesis and photosynthesis [57], [65] |
Na+/K+ exchanging ATPase | Na,K-ATPase | Actives transport of ions leading to a low sodium and a high potassium concentrations in cells [9] |
Cytochrome c oxidase subunit XV | Cox15 | Essential components of the mitochondrial respiratory chain (e.g., [121], [122]) |
Aldehyde dehydrogenase 3 | Aldh3 | Involved in the oxidation of aldehydes generated by the metabolism of a broad range of compounds, including alcohols, amino-acids, vitamins, steroids or lipids (e.g., [121], [122] |
NADH dehydrogenase I chain B | Ndh1b | |
Peroxiredoxin 6 | Prx6 | Involved in the antioxidant defense system [91] |
Triacylglycerol lipase | Tgl | Role in releasing fatty acids from the triacylglycerol storage form [79] |
Retinol dehydrogenase 1 | Rdh1 | Involved in the retinoid metabolism, vision and heat stress response [82], [83] |
Diacylglycerol O-acyltransferase | Dgat1 | Catalyzes the formation of triglycerides from diacylglycerol, using AcylCoA [79] |
Acyl-CoA oxidase | Aco | Involved in the peroxisomal beta-oxidation of fatty acids [79] |
Carbonic anhydrase | CA | Involved in CO2 transport associated with photosynthesis, respiration and calcification (e.g., [65]) |
Major yolk protein | Myp | Constitutes source of nutrients during gametogenesis [123], [124] |
Long-chain fatty-acid-CoA ligase | FadD | Catalyzes the bioactivation of fatty acids, leading to the formation of acylCoA thioesters [79] |
Actin1 | Actin1 | Involved in cell motility and cell structure |
Collagen | Coll | Involved in structural support in animal tissues; dominant in mesoglea, possibly associated with symbiosis [87] |
Galaxin | Gal | Constituent in the organic cellular matrix with a role in biomineralisation [54], [125]. |
Fibrinogen | Fib | Role in symbiosis [86] |
60S Ribosomal protein L9-like isoform | RIBOL9 | Internal control gene [105] |
Unknown transcript | Ctg1913 | Internal control gene [105] |
Putative functional role is based on http://www.ncbi.nlm.nih.gov/protein, http://expasy.org/, cited sources and references therein.